Journal: International Journal of Molecular Sciences

Article Title: Ethyl Pyruvate Decreases Collagen Synthesis and Upregulates MMP Activity in Keloid Fibroblasts and Keloid Spheroids

doi: 10.3390/ijms25115844

Figure Lengend Snippet: Effects of ethyl pyruvate on the expression of fibrotic and cytostatic markers in HDFs and KFs. ( A ) Treatment of TGF-β significantly increased the expression of collagen I/III mRNA. Treating ethyl pyruvate significantly decreased mRNA expression as the dose increased. ( B ) Similarly, treating ethyl pyruvate to KFs showed reduced collagen I/III mRNA expression (* p < 0.05).

Article Snippet: For IHC staining, the keloid spheroid sections were incubated overnight at 4 °C with primary antibodies including mouse anti-HMGB1 (Abcam), mouse anti-AKT (Abcam), mouse anti-ERK1/2 (Abcam), mouse anti-collagen type I (Abcam), mouse anti-collagen type III (Sigma-Aldrich), mouse anti-elastin (Sigma-Aldrich), and mouse anti-fibronectin (Santa Cruz Biotechnology).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Ethyl Pyruvate Decreases Collagen Synthesis and Upregulates MMP Activity in Keloid Fibroblasts and Keloid Spheroids

doi: 10.3390/ijms25115844

Figure Lengend Snippet: Effect of ethyl pyruvate on expression of fibrosis-related protein. ( A ) Western blot analysis of collagen I, III, TGF-β, and p-SMAD2/3 expression was performed; ( B ) upon densitometric analysis, collagen I, III, TGF-β, and p-SMAD2/3 protein expression levels decreased when KF cells were treated with 20mM of ethyl pyruvate (* p < 0.05).

Article Snippet: For IHC staining, the keloid spheroid sections were incubated overnight at 4 °C with primary antibodies including mouse anti-HMGB1 (Abcam), mouse anti-AKT (Abcam), mouse anti-ERK1/2 (Abcam), mouse anti-collagen type I (Abcam), mouse anti-collagen type III (Sigma-Aldrich), mouse anti-elastin (Sigma-Aldrich), and mouse anti-fibronectin (Santa Cruz Biotechnology).

Techniques: Expressing, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Ethyl Pyruvate Decreases Collagen Synthesis and Upregulates MMP Activity in Keloid Fibroblasts and Keloid Spheroids

doi: 10.3390/ijms25115844

Figure Lengend Snippet: Immunohistochemical analysis of collagen I and III, fibronectin, and elastin in ethyl pyruvate-treated keloid spheroids ( n = 3). ( A ) Representative images of collagen I and III, fibronectin, and elastin IHC staining of keloid spheroids treated with ethyl pyruvate (10, 20, and 40 mM). ( B ) Semi-quantification of protein expression is shown. Collagen I and III, fibronectin, and elastin were significantly decreased in keloid spheroids following ethyl pyruvate application. Asterisks (*) over each column signify a statistically significant difference compared to the control (0 mM, white bar) group (* p < 0.05). ( C ) Reduction in collagen I protein expression can be seen with western blot analysis.

Article Snippet: For IHC staining, the keloid spheroid sections were incubated overnight at 4 °C with primary antibodies including mouse anti-HMGB1 (Abcam), mouse anti-AKT (Abcam), mouse anti-ERK1/2 (Abcam), mouse anti-collagen type I (Abcam), mouse anti-collagen type III (Sigma-Aldrich), mouse anti-elastin (Sigma-Aldrich), and mouse anti-fibronectin (Santa Cruz Biotechnology).

Techniques: Immunohistochemical staining, Immunohistochemistry, Expressing, Western Blot